mouse anti apc3 antibody Search Results


fkn apc  (Bio-Techne corporation)
91
Bio-Techne corporation fkn apc
Fkn Apc, supplied by Bio-Techne corporation, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/mouse+anti+apc3+antibody/pm36115544-96-35-40?v=Bio-Techne+corporation
Average 91 stars, based on 1 article reviews
fkn apc - by Bioz Stars, 2026-07
91/100 stars
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mouse anti-cdc27  (Becton Dickinson)
90
Becton Dickinson mouse anti-cdc27
Mouse Anti Cdc27, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/mouse+anti+apc3+antibody/pmc00532041-102-33-36?v=Becton+Dickinson
Average 90 stars, based on 1 article reviews
mouse anti-cdc27 - by Bioz Stars, 2026-07
90/100 stars
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mouse anti anapc3  (Santa Cruz Biotechnology)
93
Santa Cruz Biotechnology mouse anti anapc3
Mouse Anti Anapc3, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/mouse+anti+apc3+antibody/bio_rxiv__2022__01__04__474939-143-8-10?v=Santa+Cruz+Biotechnology
Average 93 stars, based on 1 article reviews
mouse anti anapc3 - by Bioz Stars, 2026-07
93/100 stars
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antibody against myosin viia  (Boster Bio)
94
Boster Bio antibody against myosin viia
Antibody Against Myosin Viia, supplied by Boster Bio, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/mouse+anti+apc3+antibody/pmc12894672-349-6-12?v=Boster+Bio
Average 94 stars, based on 1 article reviews
antibody against myosin viia - by Bioz Stars, 2026-07
94/100 stars
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c3b  (Proteintech)
96
Proteintech c3b
C3b, supplied by Proteintech, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/mouse+anti+apc3+antibody/pm36709213-298-50-48?v=Proteintech
Average 96 stars, based on 1 article reviews
c3b - by Bioz Stars, 2026-07
96/100 stars
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anti cdc27  (Santa Cruz Biotechnology)
93
Santa Cruz Biotechnology anti cdc27
Anti Cdc27, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/mouse+anti+apc3+antibody/pmc05072435-339-25-26?v=Santa+Cruz+Biotechnology
Average 93 stars, based on 1 article reviews
anti cdc27 - by Bioz Stars, 2026-07
93/100 stars
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apc3  (Bethyl)
85
Bethyl apc3
Apc3, supplied by Bethyl, used in various techniques. Bioz Stars score: 85/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/mouse+anti+apc3+antibody/pm25482201-231-33-53?v=Bethyl
Average 85 stars, based on 1 article reviews
apc3 - by Bioz Stars, 2026-07
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rabbit anti apc3  (Novus Biologicals)
90
Novus Biologicals rabbit anti apc3
Rabbit Anti Apc3, supplied by Novus Biologicals, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/mouse+anti+apc3+antibody/pmc06231052-107-35-39?v=Novus+Biologicals
Average 90 stars, based on 1 article reviews
rabbit anti apc3 - by Bioz Stars, 2026-07
90/100 stars
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apc3 mouse monoclonal antibody  (Moravian Biotech)
90
Moravian Biotech apc3 mouse monoclonal antibody
PP2A-B56 and PP2A-B55 are phosphatases for Cdc20 T70. (A) Cells treated with a control RNAi oligo or a pool of B56 RNAi oligoes targeting all isoforms were arrested in nocodazole and Cdc20 immunopurified. Samples were analyzed by Western blot for Cdc20 T70p and <t>APC3</t> T447p as well as APC3 and BubR1. The degree of Cdc20 T70p and APC3 T447p was determined by normalizing to pan-Cdc20 or pan-APC3 signals. (B and C) A nocodazole lysate was prepared and treated with the indicated phosphatase inhibitors (see also ) for 5 or 15 min as indicated. Cdc20 or APC4 was immunopurified and the level of Cdc20 T70p and APC3 T447p determined. For all experiments, three independent experiments were conducted and quantified. Ctrl, control.
Apc3 Mouse Monoclonal Antibody, supplied by Moravian Biotech, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/mouse+anti+apc3+antibody/pmc08025235-106-86-91?v=Moravian+Biotech
Average 90 stars, based on 1 article reviews
apc3 mouse monoclonal antibody - by Bioz Stars, 2026-07
90/100 stars
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mouse anti-cdc27/apc3  (Becton Dickinson)
90
Becton Dickinson mouse anti-cdc27/apc3
PP2A-B56 and PP2A-B55 are phosphatases for Cdc20 T70. (A) Cells treated with a control RNAi oligo or a pool of B56 RNAi oligoes targeting all isoforms were arrested in nocodazole and Cdc20 immunopurified. Samples were analyzed by Western blot for Cdc20 T70p and <t>APC3</t> T447p as well as APC3 and BubR1. The degree of Cdc20 T70p and APC3 T447p was determined by normalizing to pan-Cdc20 or pan-APC3 signals. (B and C) A nocodazole lysate was prepared and treated with the indicated phosphatase inhibitors (see also ) for 5 or 15 min as indicated. Cdc20 or APC4 was immunopurified and the level of Cdc20 T70p and APC3 T447p determined. For all experiments, three independent experiments were conducted and quantified. Ctrl, control.
Mouse Anti Cdc27/Apc3, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/mouse+anti+apc3+antibody/pmc03575465-456-43-46?v=Becton+Dickinson
Average 90 stars, based on 1 article reviews
mouse anti-cdc27/apc3 - by Bioz Stars, 2026-07
90/100 stars
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mouse anti-apc3  (Becton Dickinson)
90
Becton Dickinson mouse anti-apc3
PP2A-B56 and PP2A-B55 are phosphatases for Cdc20 T70. (A) Cells treated with a control RNAi oligo or a pool of B56 RNAi oligoes targeting all isoforms were arrested in nocodazole and Cdc20 immunopurified. Samples were analyzed by Western blot for Cdc20 T70p and <t>APC3</t> T447p as well as APC3 and BubR1. The degree of Cdc20 T70p and APC3 T447p was determined by normalizing to pan-Cdc20 or pan-APC3 signals. (B and C) A nocodazole lysate was prepared and treated with the indicated phosphatase inhibitors (see also ) for 5 or 15 min as indicated. Cdc20 or APC4 was immunopurified and the level of Cdc20 T70p and APC3 T447p determined. For all experiments, three independent experiments were conducted and quantified. Ctrl, control.
Mouse Anti Apc3, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/mouse+anti+apc3+antibody/pm20818157-198-5-7?v=Becton+Dickinson
Average 90 stars, based on 1 article reviews
mouse anti-apc3 - by Bioz Stars, 2026-07
90/100 stars
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apc c  (Proteintech)
93
Proteintech apc c
PP2A-B56 and PP2A-B55 are phosphatases for Cdc20 T70. (A) Cells treated with a control RNAi oligo or a pool of B56 RNAi oligoes targeting all isoforms were arrested in nocodazole and Cdc20 immunopurified. Samples were analyzed by Western blot for Cdc20 T70p and <t>APC3</t> T447p as well as APC3 and BubR1. The degree of Cdc20 T70p and APC3 T447p was determined by normalizing to pan-Cdc20 or pan-APC3 signals. (B and C) A nocodazole lysate was prepared and treated with the indicated phosphatase inhibitors (see also ) for 5 or 15 min as indicated. Cdc20 or APC4 was immunopurified and the level of Cdc20 T70p and APC3 T447p determined. For all experiments, three independent experiments were conducted and quantified. Ctrl, control.
Apc C, supplied by Proteintech, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/mouse+anti+apc3+antibody/pm40307251-287-46-54?v=Proteintech
Average 93 stars, based on 1 article reviews
apc c - by Bioz Stars, 2026-07
93/100 stars
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Image Search Results


PP2A-B56 and PP2A-B55 are phosphatases for Cdc20 T70. (A) Cells treated with a control RNAi oligo or a pool of B56 RNAi oligoes targeting all isoforms were arrested in nocodazole and Cdc20 immunopurified. Samples were analyzed by Western blot for Cdc20 T70p and APC3 T447p as well as APC3 and BubR1. The degree of Cdc20 T70p and APC3 T447p was determined by normalizing to pan-Cdc20 or pan-APC3 signals. (B and C) A nocodazole lysate was prepared and treated with the indicated phosphatase inhibitors (see also ) for 5 or 15 min as indicated. Cdc20 or APC4 was immunopurified and the level of Cdc20 T70p and APC3 T447p determined. For all experiments, three independent experiments were conducted and quantified. Ctrl, control.

Journal: The Journal of Cell Biology

Article Title: Coupling of Cdc20 inhibition and activation by BubR1

doi: 10.1083/jcb.202012081

Figure Lengend Snippet: PP2A-B56 and PP2A-B55 are phosphatases for Cdc20 T70. (A) Cells treated with a control RNAi oligo or a pool of B56 RNAi oligoes targeting all isoforms were arrested in nocodazole and Cdc20 immunopurified. Samples were analyzed by Western blot for Cdc20 T70p and APC3 T447p as well as APC3 and BubR1. The degree of Cdc20 T70p and APC3 T447p was determined by normalizing to pan-Cdc20 or pan-APC3 signals. (B and C) A nocodazole lysate was prepared and treated with the indicated phosphatase inhibitors (see also ) for 5 or 15 min as indicated. Cdc20 or APC4 was immunopurified and the level of Cdc20 T70p and APC3 T447p determined. For all experiments, three independent experiments were conducted and quantified. Ctrl, control.

Article Snippet: The following antibodies were used at the indicated dilutions for Western blot: Cdc20 mouse monoclonal antibody (1:1,000; sc-13162; Santa Cruz Biotechnology; clone E7, indicated as #1), Cdc20 mouse monoclonal antibody (MAB3775; Millipore; clone AR12, indicated as #2), Cdc20 T70 and T59 phosphorylation-specific antibodies rabbit polyclonal antibody [raised against peptides CSKVQT(Tp)PSKPG and CRTPGR(Tp)PGKSS; Moravian Biotechnology; reference 22], APC3 T447p rabbit polyclonal [raised against Peptides CGKISTI(Tp)PQIQAF; Moravian Biotechnology], anti-phospho-histone H3 (Ser 10) rabbit polyclonal antibody (1:1,000; 06–570; Millipore), APC4 mouse monoclonal antibody (1:500; Moravian Biotechnology; clone CIV 1.1), APC3 mouse monoclonal antibody (1:500; Moravian Biotechnology), Mad2 rabbit polyclonal antibody (1:500; A300-300A; Bethyl Laboratories), Bub3 mouse monoclonal antibody (1:500; 611731; BD Biosciences), BubR1 rabbit polyclonal antibody (1:1,000; A300-995A; Bethyl Laboratories), and BubR1 mouse monoclonal antibody (1:500; Biotech Research and Innovation Centers; raised against TPR domain).

Techniques: Western Blot

Cdc20 T70 is dephosphorylated by BubR1 bound PP2A-B56. (A) Schematic indicating Cdc20 and PP2A-B56 binding to BubR1. The mutations in BubR1 2A are indicated. (B) Stable cell lines expressing Venus-BubR1 WT or 2A were depleted of BubR1 by RNAi and arrested in mitosis with nocodazole. Cdc20 was immunopurified from the cells and the degree of Cdc20 T70p and APC3 T447p determined. Samples were compared with similar samples from the parental cells not treated with BubR1 RNAi. (C) Cdc20, BubR1, and APC4 were immunopurified from nocodazole arrested cells and the level of Cdc20 T70p and APC3 T447p determined. The signal from the phosphospecific antibody was normalized to the pan-specific antibody for Cdc20, and for APC3 T447p the signal was normalized to APC4. For Cdc20, two different antibodies were used. For all experiments, at least three independent experiments were conducted and quantified. Ctrl, control.

Journal: The Journal of Cell Biology

Article Title: Coupling of Cdc20 inhibition and activation by BubR1

doi: 10.1083/jcb.202012081

Figure Lengend Snippet: Cdc20 T70 is dephosphorylated by BubR1 bound PP2A-B56. (A) Schematic indicating Cdc20 and PP2A-B56 binding to BubR1. The mutations in BubR1 2A are indicated. (B) Stable cell lines expressing Venus-BubR1 WT or 2A were depleted of BubR1 by RNAi and arrested in mitosis with nocodazole. Cdc20 was immunopurified from the cells and the degree of Cdc20 T70p and APC3 T447p determined. Samples were compared with similar samples from the parental cells not treated with BubR1 RNAi. (C) Cdc20, BubR1, and APC4 were immunopurified from nocodazole arrested cells and the level of Cdc20 T70p and APC3 T447p determined. The signal from the phosphospecific antibody was normalized to the pan-specific antibody for Cdc20, and for APC3 T447p the signal was normalized to APC4. For Cdc20, two different antibodies were used. For all experiments, at least three independent experiments were conducted and quantified. Ctrl, control.

Article Snippet: The following antibodies were used at the indicated dilutions for Western blot: Cdc20 mouse monoclonal antibody (1:1,000; sc-13162; Santa Cruz Biotechnology; clone E7, indicated as #1), Cdc20 mouse monoclonal antibody (MAB3775; Millipore; clone AR12, indicated as #2), Cdc20 T70 and T59 phosphorylation-specific antibodies rabbit polyclonal antibody [raised against peptides CSKVQT(Tp)PSKPG and CRTPGR(Tp)PGKSS; Moravian Biotechnology; reference 22], APC3 T447p rabbit polyclonal [raised against Peptides CGKISTI(Tp)PQIQAF; Moravian Biotechnology], anti-phospho-histone H3 (Ser 10) rabbit polyclonal antibody (1:1,000; 06–570; Millipore), APC4 mouse monoclonal antibody (1:500; Moravian Biotechnology; clone CIV 1.1), APC3 mouse monoclonal antibody (1:500; Moravian Biotechnology), Mad2 rabbit polyclonal antibody (1:500; A300-300A; Bethyl Laboratories), Bub3 mouse monoclonal antibody (1:500; 611731; BD Biosciences), BubR1 rabbit polyclonal antibody (1:1,000; A300-995A; Bethyl Laboratories), and BubR1 mouse monoclonal antibody (1:500; Biotech Research and Innovation Centers; raised against TPR domain).

Techniques: Binding Assay, Stable Transfection, Expressing